Flexible bronchoscopy may decrease respiratory muscle strength: premedicational midazolam in focus
© Tulek et al.: licensee BioMed Central Ltd. 2012
Received: 12 July 2012
Accepted: 7 September 2012
Published: 25 September 2012
Flexible bronchoscopy (FB) is a procedure accepted to be safe in general, with low complication rates reported. On the other hand, it is known that patients with pre-existing respiratory failure have developed hypoventilation following FB. In this study the effects of FB on respiratory muscle strength were investigated by measuring maximum respiratory pressures.
One hundred and forty patients, aged between 25 and 90 years, who had undergone diagnostic bronchoscopy between February 2012 and May 2012, were recruited to the study. Pre- and post-procedure maximal inspiratory pressure (MIP) and maximal expiratory pressure (MEP) were measured. A correlation between the MIP and MEP changes and patient characteristics and FB variables were investigated.
Significant decreases in both MIP and MEP values were observed following FB (p < 0.001 for both). Decreases were attributed to the midazolam used for sedation. Significant decreases in respiratory muscle strengths were observed especially in the high-dose midazolam group, compared to both low-dose and non-midazolam groups.
It was determined that respiratory muscle weakness may arise post-procedure in patients who have undergone FB, and this is constitutively related to midazolam premedication. Respiratory muscle weakness might play a role in potential hypoventilation in critical patients who undergo FB.
KeywordsBronchoscopy Midazolam Respiratory muscle strength
Flexible bronchoscopy (FB) is a procedure that is widely used in the diagnosis and management of airway and lung diseases, and is accepted to be safe in general. Data regarding bronchoscopy complications and low complication rates are mostly obtained from retrospective studies. However, the increase in therapeutic bronchoscopy applications, which are becoming more and more complex, and the bronchoscopy applications in risky patient groups cause an increase in complication rates.
Hypoventilation has been shown to develop during and after the procedure in patients who undergo bronchoscopy[3, 4]. This has mostly been linked to sedative agents in relevant studies. However, to our knowledge, the effects of bronchoscopy itself and/or sedative agents on respiratory muscle strength have yet to be studied. The aim of this study was to evaluate the effects of bronchoscopy on respiratory muscle strength, which as an important role in respiratory function and induction of cough reflex[5, 6].
One hundred and forty patients, aged between 25 and 90 years, who had undergone diagnostic bronchoscopy between February 2012 and May 2012 were recruited to the study. Patients with hemodynamic instability (heart rate below 50 bpm or above 120 bpm, systolic arterial blood pressure below 90 mmHg or above 180 mmHg), basal oxygen saturation <90%, PaCO2 > 45 mmHg, liver or kidney dysfunction, those who were diagnosed with a neuromuscular disease, had cancer with cachexia, medium (FEV1 between 50 and 80%), severe (FEV1 between 30 and 50%) or very severe (FEV1<30%) COPD, and those who could not cooperate with respiratory muscle strength test or with benzodiazepine allergy have not been included into the study. Study protocol was approved by Selcuk University Selcuklu Medical Faculty Ethics Committee. Written informed consent was obtained from all participants.
All bronchoscopy procedures were performed by the same experienced investigator in accordance with the international guidelines. In the absence of any contraindications, IM atropine (0.01 mg/kg) was given to the patients half an hour prior to the procedure. 2% lidocaine was applied topically to the inside of the mouth, pharynx, upper respiratory airways and endobronchially. As a routine application at our clinic, IV midazolam was offered to all patients by the bronchoscopist prior to the procedure and 1 mg IV midazolam was given to those who accepted the offer 3 minutes prior to the procedure. While no additional doses were given to patients who had adequate sedation during the procedure, additional doses of 1 mg were given in 2-minute intervals to patients considered to be in need by the bronchoscopist. At the end of the study, patients were retrospectively separated into three groups: no midazolam (Group A), low-dose midazolam (Group B), and high-dose midazolam (Group C).
2–4 ml/min oxygen supplementation was performed in all patients, and oxygen flow was increased in case of hypoxemia. Flumazenil was kept available for suspected cases of respiratory depression. An IV vascular access was prepared, and electrocardiogram (ECG), oxygen saturation and non-invasive blood pressure monitoring were performed throughout the procedure. Total duration of bronchoscopy, midazolam dose and adverse events during the endoscopic procedure were recorded. Cardiopulmonary complications observed during the flexible bronchoscopy were defined as the following: hypotension (systolic blood pressure (BP) <90 mmHg or mean arterial blood pressure <60 mmHg), hypertension (systolic BP >180 mmHg or diastolic BP >90 mmHg), tachycardia (HR >100/min and/or a variation of >20% from baseline value), bradycardia (HR <50/min), and oxygen desaturation (SaO2 decrease <90% for >30s). Monitoring was continued for 2 hours following the procedure. 0–100 mm visual analogue scale (VAS) was used two hours after the flexible bronchoscopy to investigate the comfort of the procedure. A higher score meant better satisfaction or less discomfort (0: worst imaginable health state, 100: best imaginable health state).
Maximum respiratory pressure measurements
A hand-held respiratory pressure meter (Micro RPM; Micro Medical, Chatham, UK) was used to measure respiratory muscle strength. Maximal inspiratory pressure (MIP) was acquired from residual volume (RV) and the maximal expiratory pressure (MEP) was acquired from total lung capacity (TLC). Measurements were taken prior to bronchoscopy before premedication and after 30 minutes following the procedure by the same investigator without any knowledge of the clinical conditions of the patients. The operator explained the procedure before beginning and showed the correct manoeuvre. MIP was measured first, followed by MEP. Patients performed 5 maneuvers in intervals of 1 minute for each measurement and the highest values (<20% variance) were recorded.
Descriptive statistics are shown as mean (±SD) or count (percentage). T-test was used to compare respiratory muscle strengths before and after bronchoscopy for all patients. T-test or One-Way ANOVA were used to compare each variable for the mean MIP and MEP changes. One-Way ANOVA and Chi-square tests were utilized in the comparison of continuous and categorical variables between various midazolam dose groups, respectively. The post-hoc comparisons of significant difference between the groups were performed using the Tukey test. The statistical significance level was set to 0.05.
Demographics and clinical and bronchoscopic characteristics of patients
All patients (n = 140)
Male gender, n (%)
Patients with COPD, n (%)
History of smoking, n (%)
Smoking pack years
Duration of bronchoscopy (min)
Patient comfort (VAS mm)
Midazolam groups, n
Complications, n (%)
Comparison of the differences in pre- and post-procedure maximum respiratory pressures (Δ: pre-post) between different patient groups
Δ MIP (pre-post)
Δ MEP (pre-post)
Mean ± SD (cmH2O)
Mean ± SD (cmH2O)
Duration of bronchoscopy (min)
The post-hoc analysis in the midazolam groups revealed no significant difference between the Groups A and B in terms of Δ MIP (p > 0.05) while Δ MIP was higher in Group C when compared to Group A (p < 0.001) and Group B (p < 0.001). There was no significant difference between Group A and Group B in terms of Δ MEP (p > 0.05). Δ MEP was higher in Group C when compared to Groups A (p = 0.04) and B (p = 0.05).
Comparison of pre- and post-flexible bronchoscopy MIP and MEP values in different midazolam dose groups
MIP (cm H2O)
MEP (cm H2O)
Comparison regarding patient demographics, bronchoscopic characteristics and pre-procedure maximum respiratory pressures among different midazolam dose groups
Group A (n: 34)
Group B (n: 66)
Group C (n: 40)
Male gender, n (%)
Patients with COPD, n (%)
Smoking pack years
Duration of bronchoscopy (min)
MIP 1 (cmH 2 O)
In this study, where -to our knowledge- the effects of FB and premedication on respiratory muscle strengths were investigated for the first time, it was revealed that midazolam, administered for sedation purposes in bronchoscopy, might affect negatively the respiratory muscle strengths, as shown with MIP and MEP measurements.
Flexible bronchoscopy is generally regarded as a safe procedure. Hypoventilation is one of the most common complications and many causes of hypoventilation related to FB have been defined. These include ventilation-perfusion mismatch related to the procedure itself, upper airway obstruction, sedation-related central respiratory depression, and increased resistance due to introduction of bronchoscope into the trachea[3, 10]. Our findings show that sedation-related respiratory muscle weakness can be included among these mechanisms.
Pulse oxymetry is utilized in many centers for monitoring hypoventilation during bronchoscopy. On the other hand, patients are routinely given oxygen during FB in many centers. Bronchoscopy guidelines recommend oxygen supplementation to maintain oxygen saturation at a minimum of 90%, because it will reduce the risk of arrhythmia during and after bronchoscopy. However, even though severe CO2 retention may occur during the procedure, oxyhemoglobin desaturation may not be observed. Chhajed et al. have performed cutaneous carbon dioxide tension (PcCO2) measurements in addition to the oxymetry in patients that underwent FB in which sedation was achieved with intermittent intravenous midazolam and 5 mg of hydrocodone, and determined an increase in PcCO2 in all but one patient. The highest PcCO2 value was significantly associated to the baseline PcCO2 (p < 0.0001) and lowest SpO2 (p = 0.016). Dreher et al. have utilized PcCO2 measurements in order to evaluate alveolar hypoventilation in patients with pre-existing respiratory failure, and determine a significant increase in PcCO2 during the procedure. In their study, while no significant difference was determined in terms of PcCO2 during FB between the groups where the patients were sedated with either midazolam alone or midazolam plus alfentanil, PcCO2 was higher compared to baseline in the midazolam alone group 120 minutes after the procedure than the midazolam plus alfentanil group. The cause for prolonged hypoventilation was considered to be midazolam due to the fact that the midazolam alone group received twice the amount of midazolam than the midazolam plus alfentanil group (4 mg vs. 2 mg). We believe that the decrease in both the inspiratory and expiratory respiratory muscle strengths, which was determined in the high-dose (0.05 ± 0.03 mg/kg) midazolam group in our study, might play an important role in hypoventilation that has been determined in the two studies mentioned above. However, because we did not measure PcCO2 in our study, we do not know whether there was an increase in the CO2 values or not, and thus, we do not know whether the determined muscle weakness has any clinical importance or not.
The effects of midazolam on respiratory muscle strength have been previously shown in experimental and clinical studies. Fujii et al. have investigated the effects of midazolam and propofol on diaphragm contractility in dogs. In that study, they have induced diaphragmatic fatigue with intermittent supramaximal bilateral electrophrenic stimulation at low or high frequency (20 and 100 Hz, respectively), and after the induction of fatigue, in order to assess the diaphragm contractility, transdiaphragmatic pressure (Pdi) and integrated electrical activity of the crural (Edi-cru) and costal (Edi-cost) parts of the diaphragm were measured. Their findings showed that midazolam caused a decrease in Pdi at both frequencies when compared to fatigued values (p < 0.05), and that Edi-cru and Edi-cost at 100 Hz stimulation during midazolam administration were below the baseline values (p < 0.05). They have also reported a lower Pdi value in the midazolam group than in the propofol group (p < 0.05).
In another study, the effects of sedative (0.1 mg/kg/h) and anesthetic (0.5 mg/kg/h) dosages of midazolam on the decrease in diaphragm contractility, fatigue (detail fatigue rating [DFR]), have been evaluated in dogs. They showed that an infusion of midazolam has caused a decrease from baseline values (p < 0.05) in Pdi at 20 and 100 Hz stimulations, and that%Edi-cru and % Edi-cost values at 100 Hz were below baseline (p < 0.05) in both sedative and anesthetic groups. They have also demonstrated that the Pdi and % Edi decrease was greater in the anesthetic dose group than in the sedative dose group (p < 0.05). Their findings show that contractility of fatigued diaphragm dose-dependently decreases with midazolam.
Molliex et al. have studied the effects of midazolam, with a dosage of 0.1 mg/kg, on total pulmonary resistance and diaphragmatic, intercostal and abdominal muscle patterns in 9 healthy volunteers. Changes in gastric pressure (ΔPga) and pleural pressure (ΔPpl) were measured in all participants, and the reduction in diaphragm contractility was evaluated with ΔPga/ ΔPpl. Midazolam was determined to increase total pulmonary resistance in sedative doses, and associatively, an increase in intercostal muscle activity was also determined; however, the diaphragmatic contribution to respiratory process was found to be decreased. This was explained as a shift from an abdominal breathing to predominantly rib cage breathing rather than a decrease in diaphragm contractility. Even though upper airway measurements were not taken in the current study, the increase in pulmonary resistance is thought to be associated with upper airway occlusion. Diazepam causing a decrease in the activity of the genioglossus muscle, which has an important role in maintaining the patency of the upper airway, supports this hypothesis.
It is known that bronchoscopy deleteriously affects pulmonary mechanics and lung volumes[10, 15]. This may arise a question regarding whether the significant reductions in maximal pressures are related to lung volumes rather than muscle weakness or respiratory muscle strength. MIP is measured at or close to RV and MEP at or close to TLC. Sometimes these measurements were performed at functional residual capacity. Although the latter may be more accurate for some studies, in that case the lung volumes should be specifically stated. In patients with abnormally high lung volumes, a low MIP may partly reflect the shortened inspiratory muscle fiber length associated with increased lung volume at RV rather than reduced inspiratory muscle strength; however, in our study all moderate to very severe COPD patients were not taken into the study and also the groups were not different regarding the number of mild COPD patients. Therefore it may be considered that the reductions in maximal pressures are probably not related to lung volumes.
Although there is limited falls in MEP values in patients who were not sedated during bronchoscopy, when Δ MEP values were compared between groups, the post-hoc analysis revealed that the difference was statistically significant only with group A and C and the decline in MEP values was higher in the high-dose midazolam group. When pre and post MEP values within each group were compared, post MEP values in group B and C were significantly lower than group A. It is notable that although post MIP values were significantly lower only in Group C, there were remarkable falls in post MEP values in all groups. The fall in post MEP values even in group A, i.e. in groups who were not sedated with midazolam might be due to exhaustion of the patient after repeated maneuvers for correct MIP measurements.
Even though the overall bronchoscopy comfort scores were quite high in our study, the fact of significantly higher VAS scores of the patients in the high-dose rather than in the low-dose midazolam group, and of the similarity of the patient comfort levels in the low-dose and no midazolam groups can be explained by the bronchoscopist’s tendency to administer low doses. It is known that objective techniques, such as electroencephalogram-based bispectral index or Ramsay sedation score, allow a more effective titration of the sedatives. In addition, it is also shown that both inter-individual and intra-individual variations are seen in the online sedation monitoring in healthy volunteers who were sedated with midazolam. This condition may explain why some patients needed high doses and some low doses of midazolam in our study although the bronchoscopist decided the appropriate sedation level in patients subjectively. In humans, midazolam is mostly metabolized by CYP3A4⁄5 isozymes to one major metabolite, 1-hydroxymidazolam and to some extent to 4-hydroxymidazolam and 1,4-dihydroxymidazolam[20, 21]. It is well known that wide inter-individual variations in hepatic and intestinal CYP3A activity are seen in the human population.
In conclusion, midazolam for premedication purposes has been determined to increase comfort in patients undergoing flexible bronchoscopy, however, causing significant decrease in respiratory muscle strength. This might increase the probability of possible complications following bronchoscopy in critical patients who have advanced COPD with limited respiratory reserves or respiratory insufficiency. Certainly there is a special need for further studies with risk patient groups and the use of high-dose midazolam.
- Facciolongo N, Patelli M, Gasparini S, Lazzari Agli L, Salio M, Simonassi C, Del Prato B, Zanoni P: Incidence of complications in bronchoscopy. Multicentre prospective study of 20,986 bronchoscopies. Monaldi Arch Chest Dis. 2009, 71 (1): 8-14.PubMedGoogle Scholar
- Jin F, Mu D, Chu D, Fu E, Xie Y, Liu T: Severe complications of bronchoscopy. Respiration. 2008, 76 (4): 429-433. 10.1159/000151656.View ArticlePubMedGoogle Scholar
- Chhajed PN, Rajasekaran R, Kaegi B, Chhajed TP, Pflimlin E, Leuppi J, Tamm M: Measurement of combined oximetry and cutaneous capnography during flexible bronchoscopy. Eur Respir J. 2006, 28 (2): 386-390. 10.1183/09031936.06.00088005.View ArticlePubMedGoogle Scholar
- Dreher M, Ekkernkamp E, Storre JH, Kabitz HJ, Windisch W: Sedation during flexible bronchoscopy in patients with pre-existing respiratory failure: Midazolam versus Midazolam plus Alfentanil. Respiration. 2010, 79 (4): 307-314. 10.1159/000267227.View ArticlePubMedGoogle Scholar
- Begin P, Grassino A: Inspiratory muscle dysfunction and chronic hypercapnia in chronic obstructive pulmonary disease. Am Rev Respir Dis. 1991, 143 (5 Pt 1): 905-912.View ArticlePubMedGoogle Scholar
- Park JH, Kang SW, Lee SC, Choi WA, Kim DH: How respiratory muscle strength correlates with cough capacity in patients with respiratory muscle weakness. Yonsei Med J. 2010, 51 (3): 392-397. 10.3349/ymj.2010.51.3.392.PubMed CentralView ArticlePubMedGoogle Scholar
- British Thoracic Society guidelines on diagnostic flexible bronchoscopy. Thorax. 2001, 56 (1): 1-21. 10.1136/thorax.56.1.1.
- Dimitriadis Z, Kapreli E, Konstantinidou I, Oldham J, Strimpakos N: Test/retest reliability of maximum mouth pressure measurements with the MicroRPM in healthy volunteers. Respir Care. 2011, 56 (6): 776-782. 10.4187/respcare.00783.View ArticlePubMedGoogle Scholar
- Evans JA, Whitelaw WA: The assessment of maximal respiratory mouth pressures in adults. Respir Care. 2009, 54 (10): 1348-1359.PubMedGoogle Scholar
- Matsushima Y, Jones RL, King EG, Moysa G, Alton JD: Alterations in pulmonary mechanics and gas exchange during routine fiberoptic bronchoscopy. Chest. 1984, 86 (2): 184-188. 10.1378/chest.86.2.184.View ArticlePubMedGoogle Scholar
- Fujii Y, Toyooka H: Midazolam versus propofol for reducing contractility of fatigued canine diaphragm. Br J Anaesth. 2001, 86 (6): 879-881. 10.1093/bja/86.6.879.View ArticlePubMedGoogle Scholar
- Fujii Y, Uemura A, Toyooka H: Midazolam-induced muscle dysfunction and its recovery in fatigued diaphragm in dogs. Anesth Analg. 2003, 97 (3): 755-758.View ArticlePubMedGoogle Scholar
- Molliex S, Dureuil B, Montravers P, Desmonts JM: Effects of midazolam on respiratory muscles in humans. Anesth Analg. 1993, 77 (3): 592-597.View ArticlePubMedGoogle Scholar
- Leiter JC, Knuth SL, Krol RC, Bartlett D: The effect of diazepam on genioglossal muscle activity in normal human subjects. Am Rev Respir Dis. 1985, 132 (2): 216-219.PubMedGoogle Scholar
- Peacock AJ, Benson-Mitchell R, Godfrey R: Effect of fibreoptic bronchoscopy on pulmonary function. Thorax. 1990, 45 (1): 38-41. 10.1136/thx.45.1.38.PubMed CentralView ArticlePubMedGoogle Scholar
- ATS/ERS Statement on respiratory muscle testing. Am J Respir Crit Care Med. 2002, 166 (4): 518-624.
- Bower AL, Ripepi A, Dilger J, Boparai N, Brody FJ, Ponsky JL: Bispectral index monitoring of sedation during endoscopy. Gastrointest Endosc. 2000, 52 (2): 192-196. 10.1067/mge.2000.107284.View ArticlePubMedGoogle Scholar
- Ramsay MA, Savege TM, Simpson BR, Goodwin R: Controlled sedation with alphaxalone-alphadolone. Br Med J. 1974, 2 (5920): 656-659. 10.1136/bmj.2.5920.656.PubMed CentralView ArticlePubMedGoogle Scholar
- Haenggi M, Ypparila-Wolters H, Hauser K, Caviezel C, Takala J, Korhonen I, Jakob SM: Intra- and inter-individual variation of BIS-index and Entropy during controlled sedation with midazolam/remifentanil and dexmedetomidine/remifentanil in healthy volunteers: an interventional study. Crit Care. 2009, 13 (1): R20-10.1186/cc7723.PubMed CentralView ArticlePubMedGoogle Scholar
- Kronbach T, Mathys D, Umeno M, Gonzalez FJ, Meyer UA: Oxidation of midazolam and triazolam by human liver cytochrome P450IIIA4. Mol Pharmacol. 1989, 36 (1): 89-96.PubMedGoogle Scholar
- Wandel C, Bocker R, Bohrer H, Browne A, Rugheimer E, Martin E: Midazolam is metabolized by at least three different cytochrome P450 enzymes. Br J Anaesth. 1994, 73 (5): 658-661. 10.1093/bja/73.5.658.View ArticlePubMedGoogle Scholar
- Wandel C, Witte JS, Hall JM, Stein CM, Wood AJ, Wilkinson GR: CYP3A activity in African American and European American men: population differences and functional effect of the CYP3A4*1B5'-promoter region polymorphism. Clin Pharmacol Ther. 2000, 68 (1): 82-91. 10.1067/mcp.2000.108506.View ArticlePubMedGoogle Scholar
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