Figure 3

Failure to suppress the lps-induced proinflammatory response of monocytes/macrophages by treg cells in moderate bronchial asthma. Monocytes were cultured as described in the legend of Figure 2. After 40 h of co-culture, LPS (50 ng/ml) was added and 24 h later cytokine production was measured by ELISA (IL-6, TNF-α) or Luminex (IL-1β). The average production of ten independent experiments ± SD of proinflammatory cytokines is shown for the three culture conditions. Significant differences are seen in the graphs between moderate asthmatic children and healthy children. Moderate asthma = white bars; healthy controls = black bars.