Figure 5

Chronic alcohol consumption impairs airway endothelial barrier function and induces inflammation in the lung. Definition of abbreviations: PGN, peptidoglycan; TNF, tumor necrosis factor. C57/B6 or TNF-deficient mice (6 mice per group) were treated with chronic alcohol consumption or saline pretreatement with or without exogenous PGN. Mice were exposed to PGN on the last day. Mouse lung endothelial permeability was determined with the Evans blue method. The permeability of lung endothelial was also assessed by evaluating the ratio of water in lung tissue. A, the scatter plots show Evans blue contents in the lung. Each dot represents the Evans blue level in lung tissue of one mouse. B, the scatter plots show the amount of water in the lung of mice presenting as percent of lung tissue. Each dot represents the amount of water in lung tissue of one mouse.