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Fig. 4 | Multidisciplinary Respiratory Medicine

Fig. 4

From: Differentiation of respiratory epithelium in a 3-dimensional co-culture with fibroblasts embedded in fibrin gel

Fig. 4

Immunohistochemical staining, pan-cytokeratin and MUC5AC. a-c Immunohistochemical staining against pan-cytokeratin and MUC5AC of respiratory epithelial cells seeded onto pure fibrin gel after 7, 14, 33 days of ALI-culture, respectively. Respiratory epithelial cells seeded onto pure fibrin gel showed a uniformly high expression of pan-cytokeratin in all cell layers. Some strongly MUC5AC positive cells are found at day 14. d-f Immunohistochemical staining against pan-cytokeratin and MUC5AC of respiratory epithelial cells seeded onto fibrin gel with embedded fibroblasts after 7, 14, 33 days of ALI-culture. Cells showed a similar pan-cytokeratin expression pattern to native trachea (i), with basal cells having a higher signal than apical cells and high signaling in the perinuclear area. Cells at all points of time carry a layer of MUC5AC positive mucus. g-i: Negative control of immunohistochemical staining against pan-cytokeratin and MUC5AC of respiratory epithelial cells seeded onto fibrin gel with embedded fibroblasts and respiratory epithelial cells seeded onto pure fibrin gel. j Native tracheal epithelium stained against pan-cytokeratin and MUC5AC, basal cells show a higher signal level of pan-cytokeratin than apical cells, the highest signaling occurs in the perinuclear area. k Negative control of immunohistochemical staining against pan-cytokeratin of native trachea. Scale: 20 μm

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