Fig. 5

Segmentation and quantification of airways and lung lobes on Micro-CT. Airway models were split into a central and a distal part, that anatomically is the intrapulmonary airway tract, leading to each defined lobe (a). Airway radius was evaluated with Micro-CT in the distal part of the airway tree of untreated and treated mice with bleomycin at 7, 14 and 21 day (c). Lungs were split into lobes by identification of the fissure lines from the CT scan. Lobes were identified as: Right Cranial Lobe (RCrL), Right Middle Lobe (RMdL), Right Caudal Lobe (RCdL), Right Accessory Lobe (RAcL) and Left Lung (LL) (b). Total lung fibrosis was evaluate on Micro-CT of treated mice with saline or bleomycin at 7, 14 and 21 day (d). Each point presents the mean ± s.e.m of 5 animals, for a total of 30 mice. Changes were compared to the saline group using one-way ANOVA followed by Dunnett’s t test for multiple comparison. *p < 0.05; **p < 0.01