Skip to main content
  • Original research article
  • Open access
  • Published:

Idiopathic pulmonary fibrosis: prognostic impact of histologic honeycombing in transbronchial lung cryobiopsy



Prognostic evaluation in idiopathic pulmonary fibrosis (IPF) may be important as it can guide management decisions, but the potential role of honeycomb changes in providing information about outcome and survival of patients with IPF, particularly if diagnosed using cryobiopsy, has not been evaluated. Aim of this study was to determinate whether a relationship exists between honeycombing on cryobiopsy and clinical/radiological picture and outcome in patients with IPF and to assess whether the same pathologic criteria that have been used to define the UIP pattern (usual interstitial pneumonia) for surgical biopsy can also be applied to cryobiopsy.


Sixty-three subjects with a multidisciplinary diagnosis of IPF and a UIP pattern on cryobiopsy were evaluated. Patients were classified into two sub-groups depending on the presence of honeycombing on histology.


The presence of honeycombing on cryobiopsy did not identify a specific phenotype of patients as it did not correlate with radiological and clinical picture and it was not associated neither with the risk of death (p = 0.1192) or with the event-free survival (p = 0.827); a higher number of samples and the presence of pleura on biopsy were instead associated with an increase in the finding of honeycombing.


The same pathologic criteria that have been used to define the UIP pattern in surgical biopsies (with honeycombing changes considered as non-mandatory for the definition of the pattern itself) can be applied to cryobiopsy samples, as the presence of these changes do not define different clinical or radiological phenotypes of patients with IPF.


Prognostic evaluation in Idiopathic Pulmonary Fibrosis (IPF) may be important as it can guide management decisions. Correlation between lung pathologic features and survival has been controversial in the past; a significant number of more recent studies have highlighted the role of fibroblastic foci (FF) as predictor of physiologic decline and mortality. However, in the literature there are no papers showing the potential role of honeycomb changes in providing information about outcome and survival of patients with IPF, particularly in patients diagnosed using transbronchial lung cryobiopsy (TLCB). The aim of this study was to determinate whether a relationship exists between pathologic honeycombing and clinical picture, radiological features and mortality in patients with a multidisciplinary diagnosis of IPF obtained with transbronchial lung cryobiopsy.


This study was submitted by the Area Vasta Romagna Ethical Committee. Prior to the procedure, written informed consent has been obtained from all subjects. Sixty-three subjects who had undergone transbronchial lung cryobiopsy (TLCB) at the Pulmonology Unit of G.B. Morgagni– L. Pierantoni Hospital in Forlì (Italy) for suspected diffuse parenchymal lung disease from January 2013 to December 2016 were retrospectively identified from the Interstitial Lung Disease (ILD) database.

All subjects had clinical information, serology testing and high-resolution computed tomography (HRCT) scans compatible with a fibrotic ILD, but insufficient elements to achieve an IPF diagnosis by current international consensus criteria; for all included patients, final multidisciplinary diagnosis was IPF after achieving a morphological picture compatible with UIP pattern on cryobiopsy. HRCT images were made within 1 month prior to the bronchoscopy and CT scans were analyzed by a dedicated radiologist (SP) under standard clinical care.

Bronchoscopies were performed as previously described [1, 2] under fluoroscopic guidance: 1.9 mm/2.4 mm cryoprobes were used (ERBE, Germany), patients were deeply sedated with intravenous propofol and remifentanil and intubated with a rigid tracheoscope, maintaining spontaneous breathing. Bronchoscopic cryobiopsy was targeted to the areas of abnormality seen on HRCT, therefore samples have been taken from either one site or multiple sites depending on the radiological pattern. When more than one site was sampled, UIP pattern had to be observed in at least one of the sampled sites. Once brought into position, the probe was cooled for approximately 5–6 s in case of 2.4 mm probe or 7–8 s in case of 1.9 mm probe, then it was retracted with the frozen lung tissue being attached on the probe’s tip. The frozen specimen was thawed in saline and then transferred to formalin for fixation. To prevent severe bleeding, we used Fogarty balloon in each procedure, placing it in the lobar bronchus near the biopsy segment and inflating it always after biopsy, then immediately deflating it in case of absence of hemorrhage or after bleeding cessation.

Biopsy specimens were fixed in formalin and embedded in paraffin. Hematoxylin and eosin (H&E) stained slides were reviewed by the standard protocol of the Morgagni Hospital clinical pathology laboratory. Specimens were reviewed under standard clinical care by two expert lung pathologists (AD and VP). The pathologic diagnosis of usual interstitial pneumonia (UIP) was made according to the criteria listed in the current guidelines [3,4,5]: for the identification of a UIP pattern, the morphologic descriptors were dense fibrosis with architectural distortion, predominant sub-pleural and/or para-septal distribution of fibrosis, fibroblastic foci, patchy involvement of lung parenchyma by fibrosis and absence of features suggesting alternative diagnoses; moreover, patients were classified into two sub-groups depending on the presence of honeycombing on histology: group A included patients with a combination of patchy fibrosis, fibroblastic foci and honeycombing; group B included patients with patchy fibrosis and fibroblastic foci, without honeycombing (Fig. 1). The exclusion criteria were: (a) known cause of pulmonary fibrosis (connective tissue diseases, occupational and/or environmental exposure, drugs, etc.); (b) no histology available; (c) pathologic findings consistent with NSIP (non-specific interstitial pneumonia), DIP (desquamative interstitial pneumonia), RB-ILD (respiratory bronchiolitis-interstitial lung disease), LIP (lymphocytic interstitial pneumonia), DAD (diffuse alveolar damage), eosinophilic granuloma, HP (hypersensitivity pneumonitis), sarcoidosis, OP (organizing pneumonia); (d) ancillary findings on histology diagnostic of secondary UIP (isolated giant cells and/or tiny granulomas in the inter-alveolar interstitium, chronic inflammatory infiltrate of the pleura and interstitium, bridging fibrosis, asbestos bodies, bronchiolocentric damage, lymphoid follicles with germinal centers, alveolar and interstitial eosinophils, IgG4 positive plasma cell infiltrate, alveolar damage with/without organizing pneumonia). Clinical information, radiological features and biopsy results were then reviewed by clinicians, radiologist and pathologists in a multidisciplinary discussion and only patients with a final diagnosis of IPF were included in the study.

Fig. 1
figure 1

Transbronchial lung cryobiopsy showing UIP pattern resulting from a combination of patchy fibrosis, fibroblastic foci and honeycombing (1a) and from a combination of patchy fibrosis and fibroblastic foci only without honeycombing (1b)

Statistical analysis

Data are presented using descriptive statistics (median value, min-max). The differences between the groups were analyzed using the Mann Whitney test or the Kruskal Wallis variance test, while the post-hoc analysis was performed, when necessary, with the Dunn multiple comparison test. Comparisons between percentages were performed with Chi-square test and Fisher’s exact test. Survival curves were estimated using the Kaplan-Meier method and compared with the log-rank test (Mantel-Cox). Patients were considered censored if they 1) were still alive when last contacted (censored at the last status date), 2) had received a lung transplant (censored at the time of the transplant). Survival time was calculated as the time since lung biopsy. Logistic regression analysis was used to identify independent variables as predictors of honeycombing. The level of statistical significance was assumed for p < 0.05. All statistical analyzes were conducted using IBM SPSS version 19.


During the study period, 63 subjects undergoing TLCB were included. Honeycombing was described on histology in 18 patients (group A, 28.6%) while in 45 patients (group B, 71.4%) honeycombing was not identified. Subject characteristics are summarized in Table 1: median age of patients at diagnosis was 64 years (range 45–78) and male were 71%; median baseline forced vital capacity (FVC) was 86.0% (range 59–136), median baseline diffusing capacity of the lungs for carbon monoxide (DLCO) was 56.0% (range 23–117).

Table 1 Clinical and radiological features of patients submitted to trans-bronchial lung criobiopsy (TLCB)

Age at diagnosis, gender distribution and lung function tests did not differ between the two pathologic groups (age: p = 0.081, Mann-Whitney test; gender: p = 0.354, Fisher’s exact test respectively; FVC: p = 0,341, Mann-Whitney test; DLCO: p = 0,089, Mann-Whitney test) (Table 1). Thirty-nine patients (61,9%) had a CT characterized by a reticular pattern with peripheral traction bronchiectasis or bronchiolectasis, no honeycombing and absence of radiological features suggesting an alternative diagnosis, with a predominant basal and sub-pleural distribution (pattern that is now called “probable UIP” according to the most recent guidelines) [3,4,5,6] (Fig. 2). In 24 patients (38,1%), the CT showed evidence of fibrosis with variable or diffuse distribution and some inconspicuous features suggestive of non-UIP pattern (which corresponds to the new term “indeterminate” from the new guidelines) [3,4,5,6] (Fig. 3). The same distribution of radiological pattern was found in the two pathologic groups (p = 0,329, Chi-square test).

Fig. 2
figure 2

Chest HRCT showing a reticular pattern with peripheral traction bronchiectasis, with a predominant basal and sub-pleural distribution (probable UIP pattern)

Fig. 3
figure 3

Chest HRCT showing a CT pattern indeterminate for UIP (fibrosis with ground-glass suggestive of non-UIP pattern)

In 11 patients (17,5%) biopsies were taken from one lobe and in 52 patients (82,5%) from two different lobes; there was no statistical difference in terms of sampling strategy between the group in which honeycombing was identified and the group with no honeycombing (p = 1,000). The choice of the site and side of biopsy was guided by HRCT and was decided before the procedure. Average number of fragments was 3 (range 1–5), with no differences between the two groups in terms of samples number (p = 0,373, Mann-Whitney test). Pleural tissue was detected in 13 cases (20,6%) (Table 1).

Logistic regression analysis was performed using the presence of honeycombing on cryobiopsy as the dependent variable; independent variables were clinical features (age at diagnosis, gender, FVC, DLCO), radiological pattern, number of samples, number of sites of sampling and presence of pleura. The number of samples and the presence of pleura were independently correlated with pathologic honeycombing: every sample increases the honeycombing probability of 136% and the absence of pleura decreases the probability of 93% (Nagelkerke R-square 0.438; number of samples: B 0.860, p 0.037, Exp-B 2.363; absence of pleura: B - 2.646 p 0.001, Exp-B 0.071).

Forty-seven subjects had received treatment for their illness during the study period: 43 patients had been treated with anti-fibrotic drugs (31 pirfenidone, 12 nintedanib), corticosteroids were administered in 20 patients, NAC (N-acetyl cysteine) in 12 patients, immunosuppressive drugs in 5 patients (4 azathioprine, 1 mofetil mycophenolate) and 16 subjects had never received treatment directed at IPF.

The median length of follow up from the time of the biopsy was 41 months; 10 patients (15.8%) died during the follow up period and none of the deaths occurred within 30 days of the procedure or was related to the procedure itself. Death and survival rate were not related with the presence of honeycombing (Table 1) (in the analysis, we censored those patients who underwent lung transplantation). We reviewed the prognosis and clinical course for all patients enrolled in this study to draw the Kaplan-Meier curve. Significant prognostic differences between the groups (with or without honeycombing on histology) were not demonstrated using log-rank Mantel-Cox test (chi square 2.428, p 0.1192, Fig. 4) and the presence of honeycombing was not significantly associated with an increased risk of death. Finally, the presence of honeycombing on histology did not influence the event-free survival, with events being 1) progression of the disease, 2) acute exacerbation and 3) death) (p = 0.827, log-rank Mantel-Cox test, Fig. 5).

Fig. 4
figure 4

Kaplan-Meier curve of patients enrolled in the study. There was no prognostic difference between the groups of patients (with or without honeycombing on histology) (log-rank [Mantel-Cox] test: chi square 2.428, p = 0.1192)

Fig. 5
figure 5

Kaplan-Meier curve of TLCB patients. There was no correlation between free-event survival and presence of honeycombing on histology (log-rank Mantel-Cox test chi square 0.047, p = 0.827)


Accurate prognostic evaluation in IPF could be important as it can guide management decisions. Over the past 20 years, several papers have reported clinical, radiological and pathologic features which may influence the outcome in ILDs. The pathologic features of UIP are characterized by a temporally and spatially heterogeneous intermix of normal lung tissue, acellular bundles of collagen, microscopic honeycombing and fibroblastic foci (myxoid matrix containing aggregates of actively proliferating and collagen-producing myofibroblasts). The correlation between lung pathology and survival has been controversial in the past [7]. Old studies of outcome and histopathology had shown that prognosis of IIPs can be determined by the extent of fibrosis and cellularity on biopsy [8,9,10]; other studies have found that the degree of alveolar space granulation tissue deposition and the extent of young connective tissue within the fibroblastic foci were the only pathologic features predictive of survival in IPF [11]. A significant number of more recent studies have highlighted the very role of fibroblastic foci as predictor of physiologic decline and mortality [12,13,14].

In IPF, most fibroblast foci are observed within honeycomb cysts; however, the presence of honeycombing is not always required for the diagnosis of UIP pattern [4, 5]. Areas of honeycomb change are composed of cystic fibrotic airspaces, which are frequently lined by bronchiolar epithelium and filled with mucin [3,4,5, 15]. In reality, morphologic analysis of honeycombed areas in IPF reveals prominent bronchiolar changes which sometimes can mimic epithelial neoplasms; this abnormal proliferation of bronchiolar structures seems to be the result of deranged proliferation of distal airways that colonize the alveolar parenchyma progressively [16]. There are no papers in the literature showing the potential role of honeycomb changes, observed in surgical lung biopsies, in providing information about outcome and survival of patients with IPF. Furthermore, to date no attempts have been made to determine whether a relationship exists between pathologic honeycombing observed in transbronchial lung cryobiopsy and clinical picture, radiological features or mortality.

The key observation in our study is that honeycombing on pathologic analysis of cryobiopsy does not correlate with outcome; as a matter of fact, the presence of honeycombing was not significantly associated with an increased risk of death and with the event-free survival (evaluated including the progression of the disease, acute exacerbations and death). These results give further support to what is reported in the guidelines: honeycombing changes are not mandatory to define the UIP pattern on biopsy samples; in fact, pathologic honeycombing does not seem to define different clinical and radiological profiles of patients with IPF. Until now, UIP pattern has been defined in the literature and in the current clinical practice through criteria and morphological features deriving only from surgical lung biopsy. Recent papers about diagnostic yield of cryobiopsy in fibrotic ILDs have directly applied these criteria to the samples obtained by cryoprobe [1, 17,18,19,20,21]; the impact of TLCB on the multidisciplinary process in patients with atypical radiological picture had been similar to that of surgical lung biopsy (SLB) [22]. These data have already supported the role of TLCB in the evaluation of patients with suspected IPF and have activated a series of efforts aimed at standardizing the procedure [23]; however, until now the prognostic significance of morphological features had not been evaluated in samples obtained by cryobiopsy.

Another important point in our study is that similarly to what happens with surgical lung biopsies, pathologic honeycombing does not even correlate with radiological pattern and clinical picture and does not seem to identify a specific clinical or radiological phenotype: for example, it is not more frequently observed in older patients with more compromised lung function or it is not more frequently described in patients with a probable UIP pattern rather than in patients with an indeterminate pattern on CT [5].

Honeycombing was not influenced by the sampling strategy: it was not more frequently observed when biopsies were taken from more than one site. This could be explained by the fact that cryobiopsy was performed in different lobes in patients with significant radiographic inter-lobar heterogeneity, while it was more frequently performed in different segments of the same lobe in patients with diffuse radiographic pattern (in the upper and the lower lobes) or in patients with a significant apical-basal gradient. However, the number of samples and the presence of pleura on biopsy were independently correlated with pathologic honeycombing. Usually 2–6 biopsies [24, 25] are taken in the common clinical practice: multiple biopsies are undertaken to reduce sampling error, even if the optimal protocol has not been standardized yet. Two samples instead of only one can increase the diagnostic yield of cryobiopsy in diffuse parenchymal lung diseases [25] and it is quite simple to accept that the number of biopsies can also contribute to a significant increase in the finding of honeycombing. Regarding the presence of pleura, ideally TLCB samples the middle third of the lung tissue between the large airways and the pleura, about 1 cm from the pleura itself [26]; however, more peripheral lung tissue, including visceral pleura, is not infrequently encountered. In previous studies, approximately 30% of the samples obtained by cryobiopsy show the presence of pleura [2, 22]. On the other hand, UIP changes are usually localized in the sub-pleural regions of the lower lobes in IPF [3,4,5,6, 27] and it is not surprising that the presence of pleura in the tissue is correlated with honeycombing itself.

Our study has some limitations. First, it was retrospective in design. However, all diagnoses have been made based on current accepted radiological and pathologic criteria and multidisciplinary discussion [3, 27]; the survival Kaplan-Meier curves have been drawled considering the presence of honeycombing on histology. We know from the literature that TLCB appears to be safer than surgical lung biopsy; our previous meta-analysis has revealed an overall mortality rate with this procedure of about 0.1% among approximately 1,000 patients [2]. The analysis of more recent data published in the literature on cryobiopsy documents 7 deaths within a month after the procedure [1, 28,29,30]. In the present study, we did not observe deaths related to acute exacerbation following the sampling procedure. The risk of acute exacerbation needs to be assessed before the procedure, particularly in case of recent worsening [31,32,33,34]: our data demonstrate that TLCB can be applicable in older patients and/or patients with more severe functional deterioration compared to patients feasible for surgery. A second limitation of our study could be that we have not described in detail the effect of pharmacological treatments on the observed results; however, the 2 groups (A and B) were homogeneous from the point of view of medication and these did not influence outcome and mortality. Another possible limitation of the study is that we included only patients with lung biopsy, therefore patients with imaging and clinical parameters insufficient for a high confidence diagnosis of IPF; our patient cohort may not replicate general population with IPF, in which a significant portion of patients do not need lung biopsy. Finally, the cellularity factor (severity and extent of cellular infiltration of the alveolar walls), fibroblastic foci profusion and inter-observer agreement between the two pathologists were not evaluated.


Despite the above limitations, this simple retrospective study can give very important information, especially as it concerns aspects that have never been taken into consideration in the several papers about transbronchial lung cryobiopsy. The identification of honeycomb areas on transbronchial lung cryobiopsy has no prognostic implications and do not define the clinical and radiological profiles of patients. Finally, we can state that the same pathologic criteria that have been used to define the UIP pattern for surgical lung biopsy samples can also be applied to the lung tissue samples obtained by cryobiopsy, with honeycombing changes considered as non-mandatory for the definition of pattern itself.



diffuse alveolar damage


desquamative interstitial pneumonia


diffusing capacity of the lungs for carbon monoxide


fibroblastic foci


forced vital capacity


hematoxylin and eosin


hypersensitivity pneumonitis


high-resolution computed tomography


Interstitial Lung Disease


idiopathic pulmonary fibrosis


lymphocytic interstitial pneumonia


N-acetyl cysteine


non-specific interstitial pneumonia


organizing pneumonia


respiratory bronchiolitis-interstitial lung disease


transbronchial lung cryobiopsy


usual interstitial pneumonia


  1. Casoni GL, Tomassetti S, Cavazza A, Colby TV, Dubini A, Ryu JH, et al. Transbronchial lung cryobiopsy in the diagnosis of fibrotic interstitial lung diseases. PLoS One. 2014;9(2):e86716.

    Article  Google Scholar 

  2. Ravaglia C, Bonifazi M, Wells AU, Tomassetti S, Gurioli C, Piciucchi S, et al. Safety and diagnostic yield of transbronchial lung cryobiopsy in diffuse parenchymal lung diseases: a comparative study versus video-assisted thoracoscopic lung biopsy and a systematic review of the literature. Respiration. 2016;91(3):215–27.

    Article  Google Scholar 

  3. Raghu G, Collard HR, Egan JJ, Martinez FJ, Behr J, Brown KK, et al. An official ATS/ERS/JRS/ALAT statement: idiopathic pulmonary fibrosis: evidence-based guidelines for diagnosis and management. Am J Respir Crit Care Med. 2011;183(6):788–824.

    Article  Google Scholar 

  4. Lynch DA, Sverzellati N, Travis WD, Brown KK, Colby TV, Galvin JR, et al. Diagnostic criteria for idiopathic pulmonary fibrosis: a Fleischner society paper. Lancet Respir Med. 2018;6(2):138–53.

    Article  Google Scholar 

  5. Raghu G, Remy-Jardin M, Myers JL, Richeldi L, Ryerson CJ, Lederer DJ, et al. Diagnosis of idiopathic pulmonary fibrosis. An official ATS/ERS/JRS/ALAT clinical practice guideline. Am J Respir Crit Care Med. 2018;5:e44–68.

    Article  Google Scholar 

  6. Piciucchi S, Tomassetti S, Ravaglia C, Gurioli C, Gurioli C, Dubini A, et al. From “traction bronchiectasis” to honeycombing in idiopathic pulmonary fibrosis: a spectrum of bronchiolar remodeling also in radiology? BMC Pulm Med. 2016;16(1):87.

    Article  Google Scholar 

  7. Katzenstein AL, Myers JL. Idiopathic pulmonary fibrosis: clinical relevance of pathologic classification. Am J Respir Crit Care Med 1998; 157 (4 Pt 1): 1301–1315.

  8. Bjoraker JA, Ryu JH, Edwin MK, Myers JL, Tazelaar HD, Schroeder DR, et al. Prognostic significance of histopathologic subsets in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 1998;157(1):199–203.

    Article  CAS  Google Scholar 

  9. Daniil ZD, Gilchrist FC, Nicholson AG, Hansell DM, Harris J, Colby TV, et al. A histologic pattern of nonspecific interstitial pneumonia is associated with a better prognosis than usual interstitial pneumonia in patients with cryptogenic fibrosing alveolitis. Am J Respir Crit Care Med. 1999;160(3):899–905.

    Article  CAS  Google Scholar 

  10. Nagai S, Kitaichi M, Itoh H, Nishimura K, Izumi T, Colby TV. Idiopathic nonspecific interstitial pneumonia/fibrosis: comparison with idiopathic pulmonary fibrosis and BOOP. Eur Respir J. 1998;12(5):1010–9.

    Article  CAS  Google Scholar 

  11. King TE, Schwartz MI, Brown K, Tooze JA, Colby TB, Waldron JA Jr, et al. Idiopathic pulmonary fibrosis: relationship between histopathologic features and mortality. Am J Respir Crit Care Med. 2001;164(6):1025–32.

    Article  Google Scholar 

  12. Nicholson AG, Fulford LG, Colby TV, du Bois RM, Hansell DM, Wells AU. The relationship between individual histologic features and disease progression in idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2002;166(2):173–7.

    Article  Google Scholar 

  13. Harada T, Watanabe K, Nabeshima K, Hamasaki M, Iwasaki H. Prognostic significance of fibroblastic foci in usual interstitial pneumonia and non-specific interstitial pneumonia. Respirology. 2013;18(2):278–83.

    Article  Google Scholar 

  14. Walsh SLF, Wells AU, Sverzellati N, Devaraj A, von der Thusen J, Yousem SA, et al. Relationship between fibroblastic foci profusion and high resolution CT morphology in fibrotic lung disease. BMC Med. 2015;13:241.

    Article  Google Scholar 

  15. American Thoracic Society, European Respiratory Society. American Thoracic Society/European Respiratory Society international multidisciplinary consensus classification of the idiopathic interstitial pneumonias. Am J Respir Crit Care Med. 2002;165(2):277–304.

    Article  Google Scholar 

  16. Chilosi M, Carloni A, Rossi A, Poletti V. Premature lung aging and cellular senescence in the pathogenesis of idiopathic pulmonary fibrosis and COPD/emphysema. Transl Res. 2013;162(3):156–73.

    Article  CAS  Google Scholar 

  17. Yarmus L, Akulian J, Gilbert C, Illei P, Shah P, Merlo C, et al. Cryoprobe transbronchial lung biopsy in patients after lung transplantation: a pilot safety study. Chest. 2013;143(3):621–6.

    Article  Google Scholar 

  18. Kropski JA, Pritchett JM, Mason WR, Sivarajan L, Gleaves LA, Johnson JE, et al. Bronchoscopic cryobiopsy for the diagnosis of diffuse parenchymal lung disease. PLoS One. 2013;8(11):e78674.

    Article  CAS  Google Scholar 

  19. Fruchter O, Fridel L, Rosengarten D, Rahman NA, Kramer MR. Transbronchial cryobiopsy in immunocompromised patients with pulmonary infiltrates: a pilot study. Lung. 2013;191(6):619–24.

    Article  Google Scholar 

  20. Pajares V, Torrego A, Puzo C, Lerma E, Gil de Bernabé MA, Franquet T. Transbronchial lung biopsy using cryoprobes (in Spanish). Arch Bronconeumol. 2010;46(3):111–5.

    Article  Google Scholar 

  21. Babiak A, Hetzel J, Krishna G, Fritz P, Moeller P, Balli T, et al. Transbronchial cryobiopsy: a new tool for lung biopsies. Respiration. 2009;78(2):203–8.

  22. Tomassetti S, Wells AU, Costabel U, Cavazza A, Colby TV, Rossi G, et al. Bronchoscopic lung Cryobiopsy increases diagnostic confidence in the multidisciplinary diagnosis of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2016;193(7):745–52.

    Article  Google Scholar 

  23. Hetzel J, Maldonado F, Ravaglia C, Wells AU, Colby TV, Tomassetti S, et al. Transbronchial Cryobiopsies for the diagnosis of diffuse parenchymal lung diseases: expert statement from the Cryobiopsy working group on safety and utility and a call for standardization of the procedure. Respiration. 2018;95(3):188–200.

    Article  Google Scholar 

  24. Poletti V, Casoni GL, Gurioli C, Ryu JH, Tomassetti S. Lung cryobiopsies: a paradigm shift in diagnostic bronchoscopy? Respirology. 2014;19(5):645–54.

    Article  Google Scholar 

  25. Ravaglia C, Wells UA, Tomassetti S, Dubini A, Cavazza A, Piciucchi S, et al. Transbronchial lung Cryobiopsy in diffuse parenchymal lung disease: comparison between biopsy from 1 segment and biopsy from 2 segments – diagnostic yield and complications. Respiration. 2017;93(4):285–92.

    Article  Google Scholar 

  26. Colby TV, Tomassetti S, Cavazza A, Dubini A, Poletti V. Transbronchial cryobiopsy in diffuse lung disease: update for the pathologist. Arch Pathol Lab Med. 2017;141(7):891–900.

    Article  Google Scholar 

  27. Leslie KO. Idiopathic pulmonary fibrosis may be a disease of recurrent, tractional injury to the periphery of the aging lung: a unifying hypothesis regarding etiology and pathogenesis. Arch Pathol Lab Med. 2012;136(6):591–600.

    Article  Google Scholar 

  28. Travis WD, Costabel U, Hansell DM, King TE Jr, Lynch DA, Nicholson AG, et al. An official American Thoracic Society/European Respiratory Society statement: update of the international multidisciplinary classification of the idiopathic interstitial pneumonias. Am J Respir Crit Care Med. 2013;188(6):733–48.

    Article  Google Scholar 

  29. Echevarria-Uraga JJ, Pèerez-Izquierdo J, Garcìa-Garai N, Gomez-Jimenez E, Aramburu-Ojembarrena A, Tena-Tudanca L, et al. Usefulness of an angioplasty balloon as selective bronchial blockade device after transbronchial cryobiopsy. Respirology. 2016;21(6):1094–9.

    Article  Google Scholar 

  30. Hagmeyer L, Theegarten D, Treml M, Priegnitz C, Randerath W. Validation of transbronchial cryobiopsy in interstitial lung disease – interim analysis of a prospective trial and critical review of the literature. Sarcoidosis Vasc Diffuse Lung Dis. 2016;33(1):2–9.

    PubMed  Google Scholar 

  31. Hutchinson JP, Fogarty AW, McKeever TM, Hubbard RB. In-hospital mortality after surgical lung biopsy for interstitial lung disease in the United States. 2000 to 2011. Am J Respir Crit Care Med. 2016;193(10):1161–7.

    Article  CAS  Google Scholar 

  32. Ernst A, Eberhardt R, Wahidi M, Becker HD, Herth FJ. Effect of routine clopidogrel use on bleeding complications after transbronchial biopsy in humans. Chest. 2006;129(3):734–7.

    Article  Google Scholar 

  33. Utz JP, Ryu JH, Douglas WW, Hartman TE, Tazelaar HD, Myers JL, et al. High short-term mortality following lung biopsy for usual interstitial pneumonia. Eur Respir J. 2001;17(2):175–9.

    Article  CAS  Google Scholar 

  34. Colella S, Haentschel M, Shas P, Poletti V, Hetzel J. Transbronchial lung cryobiopsy in interstitial lung diseases: best practice. Respiration. 2018;95(6):383–91.

    Article  Google Scholar 

Download references


AMMP (Associazione Morgagni Malattie Polmonari).


None of authors have received any funding for this manuscript.

Availability of data and materials

All data and materials used and/or analyzed during the current study are available from the corresponding author on reasonable request.

Author information

Authors and Affiliations



CR takes responsibility for the content of the manuscript, including the data and analysis and is guarantor of this paper. CR, MB and VP contributed to the conception and design of this paper. CR, MB, AUW and VP contributed to the analysis and interpretation of the data. CR, MB, AUW, CG, CG, AD, SP, SP, SM, AA, ST and VP contributed to the acquisition of data. All authors revised the manuscript for important intellectual content and provided final approval of the version to be published.

Corresponding author

Correspondence to Claudia Ravaglia.

Ethics declarations

Ethics approval and consent to participate

Subjects have given their written informed consent. The study protocol was submitted by the Area Vasta Romagna Ethical Committee and has been approved by the research institute’s committee on human research. The research was conducted ethically in accordance with the World Medical Association Declaration of Helsinki.

Consent for publication

We have obtained consent for publication from patients when applicable.

Competing interests

V.P. has served as a paid consultant to Erbe, Germany. None of all others have any potential conflicts of interest with any companies/organizations whose products or services may be discussed in this paper.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (, which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ravaglia, C., Bosi, M., Wells, A.U. et al. Idiopathic pulmonary fibrosis: prognostic impact of histologic honeycombing in transbronchial lung cryobiopsy. Multidiscip Respir Med 14, 3 (2019).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: